Heidary, Fariborz and EsmaeilLashgarian, Hamed and Karkhane, Maryam and Najar Peerayeh, Shahin (2019) MolecularDetectionof IsoniazidandRifampinResistancein MycobacteriumtuberculosisIsolatesfromLorestanProvince,Iranfrom. Arch Clin Infect Dis..
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Abstract
Background: Ariseinmultidrug-resistanttuberculosis(MDR-TB),whichisdefinedastheresistancetothetwomosteffectivefirstlinetherapeuticdrugs,Isoniazid(INH)andRifampin(RIF),threatensglobalpublichealthworldwide. Resistanceof Mycobacterium tuberculosistoINHresultsfrommutationsinseveralgenesmostcommonlyinkatGgene,andresistancetoRIFisduetomutations in rpoB gene. Therefore, rapid diagnosis of MDR-TB is of high importance in controlling the disease progress and outcome. The accuratedetectionof theresistantTBstrainscanbeacceleratedbydevelopingmoleculartests. Objectives: The aim of the present local study was to isolate MDR-TB from the patients who were the residents in the west of Iran andexaminationthefrequencyof MDR-TBbetweenpatientsof Lorestanprovinceforthefirsttimeandassessthemutationsinthe regionsrelatedtoRIF/INHresistanceviaPCRandsequencingmethods. Methods: In this study, 106 isolates of M. tuberculosis were selected in health centers of Lorestan, Iran from 2014 to 2017. After culturingM.tuberculosisisolatesonLöwenstein-Jensenmedium,classicalsusceptibilitytestingproportionalmethodagainstINHand RIFwasperformed. AfterDNAextraction,PCRandsequencingwereusedtodetectmutationsrelatedtoRIFandINHresistance. Results: Theresultsdemonstrated3.8%,0.9%,and0.9% frequencyforINH+RIF,INHandRIFresistance,respectively. Importantly,4 outof 6isolatesharboredmutationsincodon290of katGgene. Also,theseisolatescontainedmutationsincodon339of rpoBgene. NomutationwasobservedininhAgeneof M.tuberculosisisolates. Conclusions:Theresultssuggestthatmoleculartechniquescanbeusedasarapidmethodfortheidentificationofdrugresistance inclinicalisolatesof M.tuberculosis. DNAsequencinghasahighsensitivityforthedetectionof resistancemutationstoRIFandINH inMDR-TBcases.Also,theresultsshowedthatthemostfrequentresistanceassociated-mutationsoccurredincodon290ofkatGand codon339rpoBgenesegments. Keywords: Mycobacteriumtuberculosis,Isoniazid,Rifampin,MultidrugResistance
| Item Type: | Article |
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| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 18 Feb 2020 08:06 |
| Last Modified: | 18 Feb 2020 08:06 |
| URI: | http://eprints.lums.ac.ir/id/eprint/1967 |
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