Molecular identification of Echinococcus granulosus sensu lato by mitochondrial COX1 and SSU-rDNA markers in dogs in the west of Iran

Mirbadie, Seyed-Reza and Zivdari, Masoomeh and Kalani, Hamed and Vafae, Mohammad-Reza (2020) Molecular identification of Echinococcus granulosus sensu lato by mitochondrial COX1 and SSU-rDNA markers in dogs in the west of Iran. Gene Reports.

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Abstract

Abstract Echinococcus granulosus sensu lato (E. granulosus s. l.) is important in terms of the medical and veterinary sciences, especially in the tropical and subtropical regions. Stray and domestic dogs are the most important definitive hosts for this parasite. The aim of this study was to determine the prevalence of E. granulosus s. l. among stray and domestic dogs using copro-nested PCR method in the west of Iran. Accordingly, 605 faecal samples of domestic (n = 424) and stray (n = 181) dogs were collected from Kermanshah (n = 301) and Lorestan (n = 304) Provinces, in the west of Iran. Initially, the samples were concentrated using formalin-ether method and the precipitates were collected for further examination using copro-PCR method using COX1 and SSU-rDNA markers in order to detect taeniid parasites and E. granulosus s. l., respectively. Totally, taeniid parasites were observed in 38 (6.28%) samples, of which 21 (3.47%) samples in domestic dogs and 17 (2.8%) samples in stray dogs were detected. Moreover, of 38 taeniid positive samples, 21 (3.47%) samples were positive for E. granulosus s. l that 13 (2.14%) samples in domestic dogs and 8 (1.32%) samples in stray dogs were detected. In conclusion, the findings of the current study specified that the overall prevalence of echinococcosis was low (3.47%) in domestic and stray dogs in the examined areas. Since western Iran is one of the areas with high potential for transmission of E. granulosus s. l., regular monitoring of the prevalence of this disease in both the final hosts and the intermediate hosts is important at regular intervals.

Item Type: Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: samad darabian
Date Deposited: 17 Feb 2020 04:31
Last Modified: 17 Feb 2020 04:31
URI: http://eprints.lums.ac.ir/id/eprint/1962

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