A Comparison of the Effects of the Hydro-Alcoholic and Aqueous Extracts of Aloe Vera on the Proliferation of MCF7

Zarei, Leila and Abtahi Froushani, Seyyed Meysam and Abkhaz, Mohammed and Eslami, Neda (2019) A Comparison of the Effects of the Hydro-Alcoholic and Aqueous Extracts of Aloe Vera on the Proliferation of MCF7. Herbal Medicines Journal. ISSN 2538-2144

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Abstract

Background and Aim: Apart from being the most common type of cancer, breast cancer is the second leading cause of death induced by cancer among women. Chemotherapy drugs are often associated with adverse effects on patients. Common medicinal therapies for breast cancer have often short-term impacts, and the consumption of some of them leads to drug resistance. The target of this study was to determine the effects of aqueous and hydro-alcoholic extract of Aloe Vera on MCF7 cells (human breast adenocarcinoma cell line). This cell line is a largely investigated epithelial cancer cell line, which is derived from breast adenocarcinoma that has the characteristics of differentiated mammary epithelium. Materials and Methods: After culturing MCF7 cancer cells in the PRMI 1460 medium, both the aqueous and hydro-alcoholic extracts of Aloe Vera were prepared in concentrations of 20, 40, 60, 80 and 100 μg/mL from bulk solution. Subsequently, MCF7 cells were contacted with different concentrations of the prepared extract. The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) test and Acridine Orange / Propidium Iodide (AO/PI) stained were used to determine the survival rate of cells. Then, the researchers applied Doxorubicin (0.1 Mm) as positive control. The T-Student test was utilized in order to analyze the data using SPSS 21. p< 0.05 was considered as a significant level. Results: The results of MTT assay and viability showed that different concentrations of the ethanol extracts of Aloe Vera plant remarkably and dose-dependently reduced the cell proliferation of MCF7 gets (p<0.05). Conclusion: Hydro alcoholic extract could be utilized as a natural compound in the treatment of breast cancer. Keywords: Hydroalcoholic, Aloe Vera, Breast cancer, MCF7

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