Assessmentof 16srRNAMethylaseGenesAmongNon-ESBLand ESBL-ProducingKlebsiellapneumoniaeIsolates

Pakzad, Iraj and Imanieini, Mohammad and Taherikalani, Morovvat and Rahbar, Mohammad (2019) Assessmentof 16srRNAMethylaseGenesAmongNon-ESBLand ESBL-ProducingKlebsiellapneumoniaeIsolates. Arch Clin Infect Dis.

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Abstract

The aim of our study was to investigate mechanisms of aminoglycoside resistance in extended-spectrum beta-lactamases (ESBL)producingKlebsiellapneumoniae(K.pneumoniae)isolatesfromIran. Tothisend,154clinicalisolatesof K.pneumoniaewerecollected fromtwohospitalsinIlamcity,Iran. TheKirby-Bauer(agardiffusion)antibiotictestingmethodwasusedtodeterminethesusceptibilitypatternof theisolatesagainstkanamycin, gentamicin, tobramycin, netilmicinandamikacin. Aminoglycosideacetyltransferases (aac(3)-IIa, aac(6’)-Ib, and aac(3)-Ia), 16SrRNA methylase genes (armA and rmtB) and ESBL genes (blaTEM, blaSHV, and blaCTX-M) weredetectedbyPCRamplification. 59.1% (n=91)of K.pneumoniaeisolatesweredetectedESBLproducerswiththephenotypictest. Moreover, blaTEM, blaSHV and blaCTX-M were detected in 83.5% (n=76), 52.7% (n=48) and 26.4% (n=24) of the ESBL-producing isolates, respectively. Among52resistantorintermediateisolatesagainstaminoglycosides,theaac(3)-IIa,aac(6’)-IbandrmtBgenesweredetected in 55.8% (n = 29), 80.8% (n = 42) and 1.9% (n = 1) of the isolates, respectively; none of the isolates, however, had the aac(3)-Ia andarmAgenes. Therefore,theresultsshowedthehighprevalenceof aminoglycosidesresistanceintheK.pneumoniaeisolates. As observed,theacetyltransferasemodifyingenzymes(aacgenes)playedmajorrolesindeterminingthisresistance.However,therate of 16srRNAmethylasegeneswasextremelylowinK.pneumoniae. Keywords: Klebsiellapneumonia,Aminoglycosides,AntibioticSusceptibility,Extended-SpectrumBeta-Lactamases,16srRNA MethylaseGenes

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