Effect of insulin-loaded trimethyl chitosan nanoparticles on genes expression in the hippocampus of diabetic rats

Kalantarian, G and Ziamajidi, N and Abbasalipourkabir, R and Mahjub, R and Goodarzi, M.T and Saidijam, M and Soleimani, Asl, S and Jamshidi, M (2019) Effect of insulin-loaded trimethyl chitosan nanoparticles on genes expression in the hippocampus of diabetic rats. Journal of Basic and Clinical Physiology and Pharmacology.

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Abstract

Diabetes mellitus is a chronic metabolic disorder that undesirably affects both central and peripheral nervous systems through the apoptosis of neurons. Insulin and insulin-like growth factors (IGFs) inhibit apoptosis of oligodendrocytes. The objective of this study was to determine whether oral insulin in the form of nanoparticles may have similar effects to injectable insulin in increasing the gene expression of IGF1 and IGF2. Insulin-loaded trimethyl chitosan nanoparticles were prepared using the polyelectrolyte complex method and characterized for size, polydispersity index, zeta potential, drug loading, and entrapment efficiency. An in vivo study was performed in different groups of male Wistar rats with diabetes mellitus type 1 treated with insulin-loaded trimethyl chitosan nanoparticles and subcutaneous injection of trade insulin (neutral protamine Hagedorn). The hippocampus of rats were studied for the expression of IGF1 and IGF2 genes by using real-time PCR, and the fold changes in gene expression were evaluated using the 2-ΔΔCt method. The expression of IGF1 and IGF2 genes in the groups treated with nano-insulin and injected insulin were significantly higher than that in the diabetic control group (p<0.001) and meaningfully lower than that in the healthy control group. However, there was no significant difference to the treated groups. Our findings suggest that future research might provide a new formulation of drugs for treating type 1 diabetes, in the form of oral insulin.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: sobhan rezaiian
Date Deposited: 11 Dec 2019 05:49
Last Modified: 11 Dec 2019 05:49
URI: http://eprints.lums.ac.ir/id/eprint/1877

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