In vitro and ex vivo effects of Quercus infectoria extract on hydatid cyst protoscolecs

Mahmoudvand, Hossein and Mirzaei, Mojgan and Khatami, Mehrdad and Mahmoudvand, Hormoz and Kheirandish, Farnaz and Niazi, Massumeh and Sepahvand, Farnaz and Nadri*, Sedigheh (2019) In vitro and ex vivo effects of Quercus infectoria extract on hydatid cyst protoscolecs. Yafteh, 21 (3).

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Abstract

Background: This study aims to evaluate the scolicidal effects of oak (Quercus infectoria) extract on hydatid cyst protoscoleces in vitro and ex vivo. Materials and Methods: Following the collection of protoscoleces from sheep livers infected with fertile hydatid cysts from a slaughterhouse in Khorramabad, they were treated with various concentrations of oak extract (250-1000 µg/mL) for 5-40 min in vitro and ex vivo. Finally the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining). Results: The mean of the mortality of protoscoleces was 100% after 5 min incubation with the concentration of 1000 mg/ml of oak extract. On the other hand, the mean of the mortality of protoscoleces after 10 incubation with the concentration of 500 mg/ml of oak extract was 100%. However, these results were not observed in the ex vivo analysis. That is, oak extract requires a longer length of time to display potent protoscolicidal effects. Conclusion: The results of this investigation revealed that oak extract could have a significant scolicidal activity on hydatid cyst protoscoleces. However, further research, particularly in human and animal subjects, is required to reach this conclusion. Keywords: Hydatidosis, Echinococcus granulosus, extract, hydatid sand, hcolicidal

Item Type: Article
Subjects: R Medicine > RD Surgery
R Medicine > RZ Other systems of medicine
Depositing User: Samira Sepahvandy
Date Deposited: 10 Dec 2019 04:33
Last Modified: 10 Dec 2019 04:33
URI: http://eprints.lums.ac.ir/id/eprint/1874

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