Investigation of simultaneous expression of the EBV BXLF2 gene and the human PR / ER gene in Breast Carcinoma of patients referred to Tabriz International Hospital

Nadim, Elham and Ahmadizadeh*, CHangiz (2019) Investigation of simultaneous expression of the EBV BXLF2 gene and the human PR / ER gene in Breast Carcinoma of patients referred to Tabriz International Hospital. Yafteh, 21 (2).

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Abstract

Background: breast cancer is currently the most common cancer among women, and is a multi-stage disease. The EBV virus is known to be a major contributor to human cancers. The development of breast cancer in humans is often regulated by steroid hormones, such as estrogen and progesterone. The aim of this study was to simultaneously investigate the expression of the EBV BXLF2 and the human PR / ER gene in breast cancer patients referred to the l Tabriz International Hospital. Materials and Methods: this descriptive cross-sectional study was performed on 50 women with mean age of 57.3 years Genomic DNA extraction from specimens was performed using the saturated salt method. The cDNA was then synthesized from the extracted RNAs. Real-time PCR was used for the expression of the BXLF2 gene, the detection of the EBV genome, and the determination of the PR / ER gene. Results: as breast cancer progressed, the expression of ER increased significantly and with the increase in the stage of the disease, the level of expression of PR decreased significantly (p-value = 0.0043). A weak correlation was found between age and viral title. There was a significant relationship between the presence of progesterone receptor gene expression and virus load in the affected person. (P-value <0.001). Conclusion: In progesterone receptors, the expression of the receptor gene in the first three steps was approximately one-and-a-half times, which then decreased at different stages, the expression of the estrogen receptor expression increased. There was a weak correlation between age and EBV titers (P-value <0.001) Keywords: Epstein-Barr Virus, Breast Carcinoma, PR / ER Gene, BXLF2 Gene.

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