The mechanism of action of methanolic extract of Rumex alveolatus on the membranes of Candida albicans, Candida paraposilosis and Candida glabratata

Ghayour Najafabadi, Mahboubeh and Madani *, Nasim (2019) The mechanism of action of methanolic extract of Rumex alveolatus on the membranes of Candida albicans, Candida paraposilosis and Candida glabratata. Yafteh, 21 (1).

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Abstract

Background: Candidiasis is a common fungal disease that is caused by different species of genital herpes and involves various parts of the body. These infections can be fatal in light of the host's immune status. Following the advancement of science and the importance of the health sector in societies, the use of chemical drugs has increased. However, due to side effects, high prices and the complex process of production of chemical drugs, medicinal plants have been considered as possible treatments. The aim of this study was to investigate the mechanism of action of methanolic extract of Rumex alveolatus on membranes of some Candida species. Materials and Methods: First, the methanolic extracts of the stem and leaves of Roumex alveolatus were prepared by the Soxhlet method. The antifungal effect of methanolic extract was determined by the Wells and MIC method. In order to compare the mechanism of the effect of this plant with amphotericin B, the glucose value was determined by an autoanalyzer, sodium and potassium levels using a flame photometer apparatus, and amino acids were identified by HPLC in a medium containing extract of plant and candida. Results: The highest diameter of zone of inhibition of Candida yeast was methanol extract at a concentration of 250 mg / ml. The results showed that the mean concentration of sodium, potassium and glucose in different species of Candida was significantly different from that of methanol extract compared to amphotericin B. Most of the free amino acids were glutamine, threonine, and alanine. Conclusion: This herbal extract has a mechanism similar to that of amphotericin B and could be a suitable alternative for high-dose chemical drugs. However, the potential side effects of the plant need to be checked.

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