A simple method for determination of carmine in food samples based on cloud point extraction and spectrophotometric detection

Heydari, Rouhollah and Hosseini, Mohammad and Zarabi, Sanaz (2015) A simple method for determination of carmine in food samples based on cloud point extraction and spectrophotometric detection. Molecular and Biomolecular Spectroscopy 150, 150. pp. 786-791.

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Abstract

In this paper, a simple and cost effective method was developed for extraction and pre-concentration of carmine in food samples by using cloud point extraction (CPE) prior to its spectrophotometric determination. Carmine was extracted from aqueous solution using Triton X-100 as extracting solvent. The effects of main parameters such as solution pH, surfactant and salt concentrations, incubation time and temperature were investigated and optimized. Calibration graph was linear in the range of 0.04– 5.0 lg mL�1 of carmine in the initial solution with regression coefficient of 0.9995. The limit of detection (LOD) and limit of quantification were 0.012 and 0.04 lg mL�1, respectively. Relative standard deviation (RSD) at low concentration level (0.05 lg mL�1) of carmine was 4.8% (n = 7). Recovery values in different concentration levels were in the range of 93.7–105.8%. The obtained results demonstrate the proposed method can be applied satisfactory to determine the carmine in food samples.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: sobhan rezaiian
Date Deposited: 25 Sep 2016 05:23
Last Modified: 08 Nov 2017 19:47
URI: http://eprints.lums.ac.ir/id/eprint/169

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