Study Phenotype and genotype bla (IMP) and bla(VIM) metallo-β-lactamases genes and pattern antibiotic resistance Pseudomonas aeruginosa isolates from clinical samples Beheshti Hospital in the city of Qom

Ghasem Azizi*, Arash and amshidi, Leila and Mohsen, Mirzaee, (2018) Study Phenotype and genotype bla (IMP) and bla(VIM) metallo-β-lactamases genes and pattern antibiotic resistance Pseudomonas aeruginosa isolates from clinical samples Beheshti Hospital in the city of Qom. Yafte, 20 (1).

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Abstract

Background: Pseudomonas aeruginosa is a gram-negative bacillus that an opportunistic pathogen in patients with immune system disorder is known. One of the common causes of nosocomial infections is considered. Organisms oxidase positive, catalase positive, animated, and aerobic and lacks the ability to ferment carbohydrates. Group B beta-lactamase, which called MBL. Since the range generally makes antibiotics such as penicillin, cephalosporin, carbapenem broad spectrum and (with the exception of monobactams) are effective, are the clinical problem. Metallo -beta-lactamase (VIM/ IMP) are plasmid genes. The aim of this study was to evaluate the phenotype and genotype of MBL genes VIM-I and IMP-I in clinical isolates of Pseudomonas aeruginosa imipenem resistant to antibiotics. Materials and Methods:in this study by cultivating in public and differential culture and perform biochemical tests isolated for definitive confirmation and study metallobetalactamase genes from PCR was used.For review antibiotic resistance and study metallobetalactamase genes phonotype by disc diffusion. Results: Group study, Pseudomonas aeruginosa isolates were collected from Beheshti Hospital in Qom. Then MBL genes VIM-I and IMP-I after amplification with along 261 bp and 587 bp were observed on gel electrophoresis. From 100 isolates of P. aeruginosa examined 48 isolates (48%) MBLproducers. Forty eight samples was MBL producers. 19 samples (58.39%) have bands of molecular weight of 587 gene IMP and 6 samples (5.12%) have bands of molecular weight of 261 gene VIM also 48 strains of 3 samples (25.6 percent) were containing both genes .antibiotic resistance to gentamicin samples, 36 samples (74%), ciprofloxacin 30 samples (62%), ceftazidime 29 samples (61%) ceftizoxime 27 samples (58%) and imipenem 19 samples (39%) were resistant. Conclusion: Compared IMP gene alone fisher test P≤0.05 significant relationship was found while the phenotype and gene VIM alone test, fisher P≤0.05 was used, there was no significant relationship. as a result of infection and region and type of antibiotics used in the sector can affect the expression of MBL. Keywords: Pseudomonas aeruginosa, Metallo-β-lactamases, MBL, Genes VIM and IMP.

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