Phenotypic and molecular detection of ESBL producing CTX-M genes in isolated from Escherichia coli poultry with colibacillosis in Khorramabad city

Rashidian*, Ehsane and Nadali, Sakineh and Nayeb Aghaee, Seyed Mohammad and Noruzian,, Hasan (2018) Phenotypic and molecular detection of ESBL producing CTX-M genes in isolated from Escherichia coli poultry with colibacillosis in Khorramabad city. Yafte, 19 (5).

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Abstract

Background: Most strains of E.coli with multi drug resistance are major cause of colibacillosis in poultry. The economic damages caused by E.coli infections, especially in the poultry industry and also the costs of the disease treatment with antibiotics are very high annually. Therefore, the aim of this study was to investigate the prevalence of bla CTX-M genes in E.coli isolates obtained from poultry disease in Khorramabad city. Materials and Methods: In this present study, 100 E.coli isolates were collected in variable organs of birds with colibacillosis and then confirmed with biochemical tests. All the positive samples were there after investigated for the presence of CTX-M genes by PCR. Results: 4 (%4) out of 100 E.coli isolates were ESBL positive based on the results of combined disk tests. PCR analysis using the specific primers revealed that %25 contained β-lactamases genes encoding CTX-M. Conclusion: Giving the importance of E.coli infections in poultry and high prevalence of ESBL- producing strains of rapid diagnostics should used routinely to determine the strains in the laboratories. The results of this study show that the E.coli is saprophytic gastrointestinal reservoir for ESBL. This issue is very important in terms of public health and the transfer of antibiotic resistance to humans. Keywords: Colibacillosis, E.coli, ESBL, CTX-M

Item Type: Article
Subjects: R Medicine > RA Public aspects of medicine
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Depositing User: Samira Sepahvandy
Date Deposited: 26 Feb 2018 09:30
Last Modified: 26 Feb 2018 09:30
URI: http://eprints.lums.ac.ir/id/eprint/1188

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